Fig 1: CD45+ Cell Infiltration after Wounding(A–L) Frozen sections of corneas at days 1, 2, and 3 and 6 weeks after wounding were immunostained for CD45+ (red), DAPI (blue). (A–C) day 1, (D–F) day 2, (G–I) day 3 (scale bar, 200 μm), and (J–L) 6 weeks with magnified inset (scale bar, 0.5 mm). (A, D, G, and J) unwounded; (B, E, H, and K) wounded-NTC; and (C, F, I, and L) wounded-US09. (M) At days 1–3, compared to UnWnd, Wnd-NTC demonstrated a 7.1-fold increase in CD45+ immunostaining. For Wnd-NTC compared to Wnd-NTC, CD45+ staining was reduced by 46.2% (p < 0.05). At 6 weeks, compared to UnWnd, Wnd-NTC demonstrated a 3.4-fold increase in CD45+ immunostaining. Comparing Wnd-NTC to Wnd-US09, CD45+ staining was reduced by 55.2% (p = 0.06). For the grouped data, compared to UnWnd, Wnd-NTC demonstrated a 4.66-fold increase in CD45+ immunostaining (p = 0.001); comparing Wnd-NTC to Wnd-NTC, CD45+ staining was reduced by 51.0% (p < 0.01). The comparison between UnWnd and Wnd-US09 was not significant. US09 reduces CD45+ cell infiltration. N = 3 rabbits per condition for days 1–3. N = 5 rabbits per condition for the 6-week time point. N = 8 rabbits per condition for grouped data.